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Author (up) Kohler, A; Sulé-Suso, J; Sockalingum, G D; Tobin, M; Bahrami, F; Yang, Y; Pijanka, J; Dumas, P; Cotte, M; van Pittius, D G; Parkes, G; Martens, H doi  openurl
  Title Estimating and correcting mie scattering in synchrotron-based microscopic fourier transform infrared spectra by extended multiplicative signal correction Type Journal Article
  Year 2008 Publication Appl Spectrosc Abbrev Journal  
  Volume 62 Issue 3 Pages 259-66  
  Corporate Author Thesis  
  Address Centre for Biospectroscopy and Data Modelling, Norwegian Food Research Institute, Matforsk, Osloveien 1, 1430 As, Norway. kohler@matforsk.no  
  Keywords Calibration; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Cell Nucleus; Humans; Lung Neoplasms; Scattering, Radiation; Spectroscopy, Fourier Transform Infrared; Synchrotrons  
  Abstract We present an approach for estimating and correcting Mie scattering occurring in infrared spectra of single cells, at diffraction limited probe size, as in synchrotron based microscopy. The Mie scattering is modeled by extended multiplicative signal correction (EMSC) and subtracted from the vibrational absorption. Because the Mie scattering depends non-linearly on alpha, the product of the radius and the refractive index of the medium/sphere causing it, a new method was developed for estimating the Mie scattering by EMSC for unknown radius and refractive index of the Mie scatterer. The theoretically expected Mie contributions for a range of different alpha values were computed according to the formulae developed by Van de Hulst (1957). The many simulated spectra were then summarized by a six-dimensional subspace model by principal component analysis (PCA). This subspace model was used in EMSC to estimate and correct for Mie scattering, as well as other additive and multiplicative interference effects. The approach was applied to a set of Fourier transform infrared (FT-IR) absorbance spectra measured for individual lung cancer cells in order to remove unwanted interferences and to estimate ranges of important alpha values for each spectrum. The results indicate that several cell components may contribute to the Mie scattering.  
  Publisher Place Editor  
  Language eng Summary Language Orig Title  
  Series Editor Series Title Abbrev Series Title  
  Series Volume Series Issue Edition  
  Issn 0003-7028 Isbn Medium  
  Area Expedition Conference  
  Notes PUBMED: 18339231; Research Support, Non-U.S. Gov\'t Approved no  
  Location Torfinn Nome (torfinn.nome@umb.no)  
  Call Number Cigene @ torfinn.nome @ Serial 1547  
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