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Author  |
Janbu, Astrid Oust; Møretrø, Trond; Bertrand, Dominique; Kohler, Achim |
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Title |
FT-IR microspectroscopy: a promising method for the rapid identification of Listeria species |
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Letter |
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Year |
2008 |
Publication |
FEMS Microbiol Lett |
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Volume |
278 |
Issue |
2 |
Pages |
164-70 |
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Keywords |
Algorithms; Bacterial Typing Techniques; Listeria; Pilot Projects; Reproducibility of Results; Spectroscopy, Fourier Transform Infrared |
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Abstract |
This work presents a pilot study to investigate the potential of fourier transform infrared (FT-IR) microspectroscopy for rapid identification of Listeria at the species level. Using this technique, FT-IR spectra were acquired from 30 strains from five Listeria species. The FT-IR spectra were analysed using stepwise canonical discriminant analysis and partial least-squares regression in a stepwise identification scheme. The results showed that 93% of all the samples were assigned to the correct species, and that 80% of the Listeria monocytogenes strains were correctly identified. In comparison, 100% of the samples, including the L. monocytogenes samples, were correctly identified using spectra acquired by FT-IR macrospectroscopy. The results show that FT-IR microspectroscopy has potential as a rapid screening method for Listeria, which is especially valuable for the food industry. |
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Language |
eng |
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Issn |
0378-1097 |
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Notes |
PUBMED: 18053065; Research Support, Non-U.S. Gov\'t |
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no |
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Location |
Torfinn Nome (torfinn.nome@umb.no) |
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Call Number |
Cigene @ torfinn.nome @ |
Serial |
1551 |
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